In vitro flowering, somatic embryogenesis and regeneration in Boerhaavia diffusa Linn. - A medicinal plant

In vitro callogenesis was achieved from leaf explants of Boerhaavia diffusa Linn. on basal medium supplemented with different concentrations of BAP, NAA, Kn and IAA. Regeneration of shoot buds were observed on MS medium supplemented with BAP and NAA at different concentrations. In some of the replicates the shoot buds were elongated and terminated in flower buds. In vitro flowering occurred within two weeks of subculture. The highest percentage of flowering was obtained on medium supplemented with BAP (2 mg l ). Excised shoots after transferring to rooting liquid medium (0.5-2.0 mg l IAA or NAA) roots were 1 1 initiated. The regenerated plantlets have been successfully established in vermiculite soil. Subsequently, they were shifted to the field conditions. Regenerated plantlets resemble the parent plants morphologically and cytologically. Histological study of the cultures showed the presence of meristemoids, somatic embryos and embryoids.